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The most abundant is 16O, with a small percentage of 18O and an even smaller percentage of 17O. Oxygen isotope analysis considers only the ratio of 18O to 16O present in a sample. The calculated ratio of the masses of each present in the sample is then compared to a standard, which can yield information about the temperature at which the sample was formed – see Proxy climate for details. The addition of more energy is required to vaporize H O than H O, and H O liberates more energy when it condenses. In addition, H O tends to diffuse more rapidly. Because H O requires less energy to vaporize, and is more likely to diffuse to the liquid surface, the first water vapor formed during evaporation of liquid water is enriched in H O, and the residual liquid is enriched in H O.

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As colder temperatures spread toward the equator, water vapor rich in 18O preferentially rains out at lower latitudes.

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